Back to Explorer

Development of Therapeutic Protein Biosimilars: Comparative Analytical Assessment and Other Quality-Related Considerations Guidance for Industry

FinalCenter for Biologics Evaluation and Research Center for Drug Evaluation and Research09/09/2025

Description

This guidance describes the Agency’s recommendations on the design and evaluation of comparative analytical studies intended to support a demonstration that a proposed therapeutic protein product is biosimilar to a reference product licensed under section 351(a) of the Public Health Service Act (PHS Act). Additionally, this guidance is intended to provide recommendations to sponsors on the scientific and technical information for the chemistry, manufacturing, and controls (CMC) portion of a marketing application for a proposed product submitted under section 351(k) of the PHS Act. This guidance finalizes and replaces the draft guidance of the same title issued on May 22, 2019, and replaces the final guidance “Quality Considerations in Demonstrating Biosimilarity of a Therapeutic Protein Product to a Reference Product” issued on April 30, 2015.

Scope & Applicability

Product Classes

5
Therapeutic Protein Biosimilars

Guidance focuses on the development and comparative analytical assessment of these products.

Interchangeable Biosimilar

A biosimilar product determined to be interchangeable with the reference product.; may be substituted for the reference product without the intervention of the prescribing health care provider

Biosimilar

Biological product shown to be highly similar to an FDA-licensed reference product; Biological product demonstrated to be highly similar to a reference product

Reference product

single biological product licensed under section 351(a) of the PHS Act; U.S.-licensed reference product used for comparative analytical assessment; analytical studies that demonstrate that the proposed product is highly similar to the reference product

Therapeutic Protein

Products produced in microbial cells or cell lines; Complex molecules manufactured in living cells

Stakeholders

1
Sponsor

Entity responsible for submitting applications under section 524B

Regulatory Context

Attributes

7
Physicochemical Properties

Factor for consideration in analytical assessment.

Protein Sequence

some attributes may be highly critical (e.g., protein sequence) but not amenable to quantitative analysis

Critical Quality Attribute

The degree of uncertainty can impact the critical quality attribute (CQA) risk ranking

Lot-to-lot variability

Inherent heterogeneity in protein products

Posttranslational Modifications

Modifications imparted to the protein product

Glycosylation

Nature, location, and levels of sugar chains

Potency

Measurement of potency for biological products

Identified Hazards

Hazards

2
Adventitious agent safety

CMC section provides information on adventitious agent safety

Adventitious agents

Safety concern regarding viral contamination

Related CFR Sections (2)

See Also (7)