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Clinical Pharmacology Considerations for Antibody-Drug Conjugates Guidance for Industry: Guidance for Industry

FinalCenter for Drug Evaluation and Research Center for Biologics Evaluation and Research03/01/2024
Dose- and exposure-response analysispharmacokineticspharmacodynamicsADMEImmunogenicity

Description

This guidance provides recommendations to assist industry and other parties involved in the development of antibody-drug conjugates (ADCs) with a cytotoxic small-molecule drug or payload. Specifically, this guidance addresses the FDA’s current thinking regarding clinical pharmacology considerations and recommendations for bioanalytical methods, dosing strategies, dose- and exposure-response analysis, intrinsic factors, QTc assessments, immunogenicity, and drug-drug interactions (DDIs). The principles discussed in this guidance might not be applicable to the development of other types of ADCs (e.g., ADCs with payloads other than cytotoxic small molecule drugs and/or for indications other than oncology).

Key Topics

Terms and concepts identified from this document

Scope & Applicability

Product Classes

2
Antibody-Drug Conjugates

Specific type of therapeutic protein discussed for DDIs; Focus of biologics DDI evaluation.; ADCs consist of a small molecule drug component conjugated to an antibody.

Combination Product

Products combining drug, device, or biological constituents; Generally recommended for Enhanced Documentation; Requires 14971-based framework incorporating ICH Q9; A drug-device combination where the device constituent part detects ingestion.

Regulatory Context

Regulatory Activities

2
Biologics License Applications

BLA application type eligible for reduced fees.

DDI studies

Drug-drug interaction studies to measure unconjugated payload.

Attributes

2
Drug to antibody ratio

DAR of at least one for an ADC

Drug-to-Antibody Ratio

ADC average DAR can be used as one constituent part in the analysis.

Technical Details

Substances

7
Cytotoxic small-molecule drug

ADC payload component

Immunosuppressors

Medications that may affect ADC PK via immunogenicity.

unconjugated payload

Constituent part of the ADC that impacts the efficacy and safety profile.; The small molecule component of the ADC.

pharmacologically active metabolite

Metabolite from the metabolism of the unconjugated payload.

Cytochrome P450 2D6

Metabolic enzyme (CYP2D6) impacting clearance.

Breast cancer resistant protein

Transporter (BCRP) impacting pharmacokinetic clearance.

Fc-gamma receptors

Receptors affecting antibody-dependent cellular cytotoxicity.

Testing Methods

7
Bioanalytical methods

Recommendations for clinical pharmacology considerations

Physiologically based pharmacokinetic modeling

appropriate to demonstrate BA or BE for complex products

Bioanalytical Method Validation

All bioanalytical methods should be validated and reported.

QTc assessments

Measuring the unconjugated payload and pharmacologically active metabolites is usually sufficient.

exposure-response analyses

Analyses conducted for safety and efficacy with the ADC and its constituent parts.

population pharmacokinetic analysis

Used to assess the effects of organ impairment on the unconjugated payload.

Anti-drug antibodies assay

Confirmatory assessment for detecting ADAs.

Processes

2
In vitro DDI risk assessment

Assessment for the unconjugated payload as inhibitor or inducer.

ADME

Absorption, distribution, metabolism, and excretion properties evaluated for extrapolation.

Clinical Concepts

5
QTc assessments

Evaluation of cardiac safety in ADC development

Immunogenicity

Assessment of the immune response to the biological product

Drug-drug interactions

Evaluation of DDIs for ADCs

organ impairment

Specific subsets of patients in pivotal studies.; Subsets of patients requiring dedicated dosing studies.

QT prolongation

Potential safety concern/adverse effect of some antiemetic drugs.

ICH References (3)

ICH M10

Drug concentrations in study samples should be measured in accordance with ICH M10, Bioanalytical Method Validation and Study Sample Analysis.; Bioanalytical method validation and study sample analysis

ICH E14

Clinical Evaluation of QT/QTc Interval Prolongation.

ICH S7B

Nonclinical Evaluation of the Potential for Delayed Ventricular Repolarization

Related CFR Sections (1)

Related MFDS Guidelines

Korean regulatory guidelines covering similar topics

See Also (6)

Clinical Pharmacology Considerations for Antibody-Drug Conjugates Guidance for Industry: Guidance for Industry | Guideline Explorer | BioRegHub