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Clinical Pharmacogenomics: Premarket Evaluation in Early-Phase Clinical Studies and Recommendations for Labeling

FinalCenter for Biologics Evaluation and Research Center for Devices and Radiological Health Center for Drug Evaluation and Research01/28/2013
PharmacogenomicsBlindingPharmacokineticsPharmacodynamicsData IntegrityAnalytical validation

Description

This guidance is intended to assist the pharmaceutical industry and other investigators engaged in new drug development in evaluating how variations in the human genome, specifically DNA sequence variants, could affect a drug’s pharmacokinetics (PK), pharmacodynamics (PD), efficacy, or safety. The guidance provides recommendations on when and how genomic information should be considered to address questions arising during drug development and regulatory review.

Key Topics

Terms and concepts identified from this document

Scope & Applicability

Product Classes

4
Biological products

development program for drug and biological products

In Vitro Companion Diagnostic Devices

Devices used to identify patients eligible for specific drug treatments

companion diagnostic

validate the companion diagnostic across cancer types

in vitro PGx test

An in vitro PGx test would be considered a companion diagnostic device

Stakeholders

1
sponsors

Target audience for recommendations on assessment of overall survival

Regulatory Context

Regulatory Activities

5
New drug development

process of evaluating genomic variations

regulatory approval

therapeutic products under review for approval

CDRH approval

required for tests used to select patients or dosing regimens

marketing approval

Trials conducted to support approval of drugs

Investigational Device Exemption

A medical device submission type (IDE).

Document Types

7
Labeling

Cybersecurity information should be included in device labeling

CLINICAL PHARMACOLOGY

Section containing detail about drug interaction studies.

BOXED WARNING

Clinically significant increases in blood pressure should be described in sections like BOXED WARNING

Prescribing information

Changes made to add a package type term to labeling and prescribing information; Document where package type terms must be consistent with labeling

product labeling

Must conform to requirements of 21 CFR 107.10-107.30.

informed consent form

the terms informed consent form and informed consent document are used interchangeably; Document used to provide information to prospective subjects; The entire document used to obtain subject participation agreement

study report

Document describing procedures followed and samples retained

Attributes

8
Benefit-risk profile

Assessment for both mother and child

genotype information

information for a patient used for dosing

metabolizer phenotypes

ultrarapid, extensive, intermediate, and poor phenotypes for CYP2C19

Body surface area

covariate associated with sex affecting device performance

Cmax

Cmax may be more informative for safety

AUC

AUC or Cmin may correlate with efficacy

ultra-rapid metabolizers

genetically defined subgroup for comparative PK

poor metabolizers

genetically defined subgroup for comparative PK

Technical Details

Substances

10
DNA

reacting with DNA bases

Abacavir

ABC (bid)+ 3TC + EFV

VKORC1

genetic variant affecting warfarin response; genotype information used for dosing

CYP2C9

enzyme induced via activation of the PXR; Cytochrome P450 enzyme

RNA

Examples of GT products can include purified nucleic acid such as RNA.

warfarin

Example drug with multiple serious drug interactions.

cetuximab

KRAS mutations with cetuximab and panitumumab indicated for colon cancer

panitumumab

KRAS mutations with cetuximab and panitumumab indicated for colon cancer

CYP2C19

enzyme induced via activation of the PXR; Cytochrome P450 enzyme

CYP2D6

enzyme generally considered not inducible by drugs; Specific enzyme used for extrapolation of DDI results.; Cytochrome P450 enzyme

Testing Methods

9
Prospective DNA sample collection

collection of samples for genomic analysis

INR

International Normalized Ratio used for warfarin dose modification

HLA-B*5701 screening

prospective screening to avoid abacavir-induced HSR

Hardy-Weinberg equilibrium

method to filter genetic markers

Bonferroni correction

method to control for multiplicity in genomic data

Physiologically based pharmacokinetic models

Models that may aid in assessing the effect of CYP3A modulators.

genome-wide association investigations

investigate the causes of lack of efficacy using exploratory genome-wide association investigations

high-throughput sequencing

data generated from higher-throughput technologies; technology platform for characterizing allelic variations

genome-wide arrays

high-throughput platform for uncharacterized pharmacology

Processes

3
Premarket Evaluation

evaluation in early-phase clinical studies

Pharmacokinetics

Dosing considerations for pregnant women in trials; Study of drug movement in the body, which may change during pregnancy.

Pharmacodynamics

Study of drug effects, which may be impacted by physiological changes in pregnancy.

Clinical Concepts

10
maximum tolerated dose

critical consideration in phase 1 oncology studies

VKORC1

gene encoding vitamin K epoxide reductase affected by polymorphisms

CYP2C19

polymorphic enzyme affecting clopidogrel metabolism

HLA-B*5701

genetic allele associated with hypersensitivity reactions

Abacavir hypersensitivity reaction

adverse reaction observed in clinical trial patients

Adverse drug reactions

Information to be included in labeling for the neonate.

Poor metabolizers

subjects with polymorphic enzymes who should not be enrolled in certain drug-drug interaction studies

Adverse reactions

Increased risk when daily wear lenses are worn overnight

colon cancer

indicated for colon cancer

QT prolongation

Potential safety concern/adverse effect of some antiemetic drugs.

Identified Hazards

Hazards

2
dose-limiting toxicity

risk associated with genetically mediated alteration in metabolism

stent thrombosis

risk increased in carriers of LOF CYP2C19 alleles

ICH References (1)

ICH E15

Definitions for Genomic Biomarkers, Pharmacogenomics, Pharmacogenetics, Genomic Data and Sample Coding Categories

Related CFR Sections (1)

  • 21CFR312.21§ 312.21 Phases of an investigation.

    An IND may be submitted for one or more phases of an investigation. The clinical investigation of a previously untested drug is generally divided into three phases. Although in general the phases are conducted sequentially, they may overlap. These three phases of an investigation are a follows:Read full regulation →

Related MFDS Guidelines

Korean regulatory guidelines covering similar topics

See Also (8)

Clinical Pharmacogenomics: Premarket Evaluation in Early-Phase Clinical Studies and Recommendations for Labeling | Guideline Explorer | BioRegHub