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Bispecific Antibody Development Programs Guidance for Industry

FinalCenter for Biologics Evaluation and Research Center for Drug Evaluation and Research05/24/2021
ImmunogenicityCMC

Description

This guidance provides recommendations to assist industry and other stakeholders involved in the development of bispecific antibodies. In addition to general considerations, the guidance provides recommendations for specific regulatory, quality, nonclinical, and clinical considerations for bispecific antibody development programs. Although this guidance is intended for bispecific antibodies, the principles discussed in this guidance may also inform the development of other types of bispecific protein products and multispecific products. This guidance does not discuss development considerations for other multitarget therapies that are antibody cocktails, polyclonal antibody products, or combinations of monoclonal antibodies.

Key Topics

Terms and concepts identified from this document

Scope & Applicability

Product Classes

3
Multispecific Product

Therapeutic product that targets three or more antigens

Monoclonal Antibody

Anti-TNF-alpha drug substance and drug product

Bispecific Antibody

Genetically-engineered, recombinant antibodies that consist of two distinct binding domains; A single molecule antibody that targets two different antigens or epitopes.

Stakeholders

3
Sponsor

Entity responsible for submitting applications under section 524B

Stakeholders

Entities involved in the development of bispecific antibodies

Industry

Guidance for Industry

Regulatory Context

Regulatory Activities

1
First-in-human dose

Selection of initial dose for clinical trials.

Attributes

1
Affinity

On- and off-rates of each arm for its target.

Technical Details

Substances

1
Soluble cytokines

Targets for certain categories of bispecific antibodies.

Testing Methods

1
In vitro assays

Adventitious virus testing using indicator cell lines; Assays performed on permissive cells for MCB and virus seeds.

Processes

4
Aseptic Processing

implied manufacturing process under QS regulation

Clinical Pharmacology Studies

Specific clinical considerations mentioned in the table of contents

Clinical Development

The purpose of this guidance is to help sponsors in the clinical development of drugs.

Nonclinical Development

Scientific considerations for bispecific antibody programs

Clinical Concepts

5
Immunogenicity

Assessment of the immune response to the biological product

Inflammatory Conditions

Conditions treated by therapeutic monoclonal antibodies

Infectious Diseases

Conditions treated by therapeutic monoclonal antibodies

Autoimmune Diseases

Conditions treated by therapeutic monoclonal antibodies

Cancer

target disease for the combination drugs

Identified Hazards

Hazards

1
Cytokine release

Risk associated with homodimers of anti-CD3 engaging arms.

Standards & References

Specifications

1
Potency

Specific ability or capacity of the product to effect a given result

ICH References (3)

ICH S9

Nonclinical Evaluation for Anticancer Pharmaceuticals; Guideline regarding pharmaceuticals for advanced cancer.

ICH S6(R1)

Preclinical Safety Evaluation of Biotechnology-Derived Pharmaceuticals; guidance regarding nonclinical immunotoxicity assessment of biopharmaceuticals

ICH M4E(R2)

The Common Technical Document (CTD)—Efficacy.

Related CFR Sections (1)

Related MFDS Guidelines

Korean regulatory guidelines covering similar topics

See Also (8)

Bispecific Antibody Development Programs Guidance for Industry | Guideline Explorer | BioRegHub